MUSIAL is a web platform that computes prokaryotic genome, gene and protein sequence alignments from variant call datasets (using both SNVs and Indels) derived from multiple samples of one species. Scientists can assess the variability of a species on the genome, gene, and protein sequence level, e.g., study which positions in the species exhibit large or no variability, identify genes with large variability, which samples agree on which proteoforms, etc. The platform is designed to support the detection of features of interest for downstream analyses. It provides multiple data export options for FASTA as well as tabular formats.
Visit MUSIAL here: https://musial-tuevis.cs.uni-tuebingen.de/
For a stand-alone version of MUSIAL visit our GitHub site: https://github.com/Integrative-Transcriptomics/MUSIAL/tree/v2.1-dev
For a tutorial, visit the following YouTube video: https://www.youtube.com/watch?v=ZMpdVQh64dk